| 摘要: |
| 为研究重组溶葡萄球菌酶对奶牛子宫内膜炎的疗效，以青霉素钠、土霉素为对照药，子宫内灌注给药，分别在河北、内蒙古、广州、上海奶牛场试验，共治疗急、慢性子宫内膜炎病例359头，抽检249头奶牛子宫分泌物进行细菌学分离鉴定与统计分析，大肠杆菌、金黄色葡萄球菌、链球菌、化脓棒状杆菌是4种检出率较高的病原菌。重组溶葡萄球菌酶治疗组与土霉素、青霉素钠比较，按400、800、1200U/次的三个治疗浓度剂量，隔日投药1次，连续用药3次，800、1200U/次的浓度剂量显效率超过80%，显著优于青霉素钠的疗效（p<0.01），与土霉素注射液组相当。追加用药次数可进一步提高奶牛子宫内膜炎的痊愈率。结果显示：重组溶葡萄球菌酶能有效治疗由金黄色葡菌球菌、链球菌、化脓棒状杆菌等革兰氏阳性菌感染的子宫内膜炎，治愈后受孕率在70%左右。尤其是对抗生素治疗无效的慢性子宫内膜炎同样具有很好的疗效。因此，用重组溶葡萄球菌酶治疗奶牛子宫内膜炎安全、高效。 |
| 关键词: 重组溶葡萄球菌酶 奶牛 子宫内膜炎 疗效 |
| DOI： |
| 投稿时间：2008-10-22修订日期：2008-12-22 |
| 基金项目:国家高技术研究发展计划（863计划） |
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| The Study of Recombinant lysostaphin on Treatment Trial of Endometritis in Dairy Cattle |
| zhangjien |
| (Shanghai Hi-Tech United Bio-Technological Research & Development Co. Ltd,) |
| Abstract: |
| To study the treatment of the rlysostaphin on endometritis of dairy cow, 359 dairy cows clinically diagnosed with either acute or chronic endometritis were selected from the dairy farm in Hebei, Inner Mongolia, Guangzhou and Shanghai for the treatment by uterine infusion, using penicillin sodium and oxytetracycline as control groups. Uterine secretions of these dairy cows were collected for bacteriological isolation and identification. Of 249 testing dairy cows, statistical analysis showed that E. coli, Staphylococcus aureus, Streptococcus and purulent Corynebacterium were the main pathogens with higher detection rate. The testing group treated with 800、1200U rlysostaphin every one day for 3 times showed the recovery rate at 80%, similar to that of the groups treated by oxytetracycline and significantly higher than that of the groups treated by penicillin. The recovery rate of endometritis improved with additional lysostaphin administration. The rate of pregnancy was 70% which is improved significantly after the treatment of rlysostaphin. For chronic endometritis of dairy cows resistant to the conventional antibiotics treatment, the efficacy of the rlysostaphin treatment remained. In summary, rlysostaphin can be used safely and efficiently to treat the endometritis of cows. |
| Key words: recombinant lysostaphin dairy cattle endometritis curative effect |
