| 摘要: |
| 建立了牛奶中硫酸头孢喹肟残留超高效液相色谱-串联质谱检测方法(UPLC-MS/MS)。选取18头刚进入干乳期的健康奶牛，随机分为 6 个组，分别于预产期前的42、35、28、21、14和7 d进行乳房注入硫酸头孢喹肟乳房注入剂（3 g∶150 mg/支），只用药一次，产犊后采集奶样。结果表明：预产期前7 d给药，奶牛产犊 72 h后采集的奶样中头孢喹肟残留量降至MRL（20 μg/kg）附近；预产期前14 d和21 d给药，奶牛产犊12 h后采集的奶样中头孢喹肟残留量降至MRL以下，均低于检测限；预产期前 28、35 和 42 d给药，所有采集奶样的头孢喹肟残留量均低于检测限。建议在预产期前 7、14 和 21 d 给药硫酸头孢喹肟乳房注入剂的弃奶期分别为产犊后5、1和0 d。 |
| 关键词: 头孢喹肟 牛奶 残留 超高效液相色谱-串联质谱法 |
| DOI： |
| 投稿时间：2015-02-05修订日期：2015-04-27 |
| 基金项目: |
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| Residual Study of Cefquinome Sulfate Intramammary Infusion (Dry Period) in Milk |
| 魏占勇 |
| (HeBei Yuanzheng Pharmaceutical.,Ltd) |
| Abstract: |
| The high performance liquid chromatography and tandem mass spectrometry method (UPLC-MS/MS) was established for the residual study of cefquinome sulfate in milk.18 healthy cows that just entering the dry period were selected and were randomly divided into 6 groups. The 6 groups of cow were respectably administrated cefquinome sulfate intramammary infusion (dry period) once in mammary region at the dose of 3 g for each region and their milk were collected after calving. The results demonstrated that: administrated 7 days before preterm birth, concentration of cefquinome in milk collected 72h after calving decreased to about MRL; administrated 14 and 21 days before preterm birth, concentration of cefquinome in milk collected 12h after calving dropped below MRL, all below the detection limit; administrated 28、35 and 42 days before preterm birth，concentration of cefquinome in all the milk were below the detection limit. It is suggested that the milk withholding period were respectably 5, 1 and 0 days that administrated cefquinome sulfate intramammary infusion7, 14 and 21 days before preterm birth. |
| Key words: cefquinome milk residue UPLC-MS/MS |
