| 摘要: |
| 建立了测定醋酸氯己定含量的高效液相色谱法。以C18（4.6 mm×150 mm, 5 μm）为色谱柱，0.02 mol/L磷酸二氢钾溶液（pH2.6）-乙腈（70∶30）为流动相，流速1 mL/min，检测波长254 nm。结果显示，醋酸氯己定在0.5~198 μg/mL浓度范围内，浓度对峰面积的线性关系良好（r=0.999 98），平均回收率为96.40%（n=9）。实验表明，该方法灵敏、准确，可以较好地控制醋酸氯己定子宫灌注剂的质量。 |
| 关键词: HPLC法 醋酸氯己定 含量测定 |
| DOI： |
| 投稿时间：2009-12-29修订日期：2010-04-05 |
| 基金项目: |
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| Determination of Chlorhexidine Acetate in Uterus Perfusion Agent by HPLC |
| zhouyanfei |
| (bjsys) |
| Abstract: |
| An HPLC method was established for the determination of chlorhexidine acetate in uterus perfusion agent. The analysis was carried on a column of C18(4.6 mm×150 mm, 5 μm) , using 0.02 mol/L KH2PO4 (pH2.6)-acetonitrile（70∶30）as the mobile phase with a flow rate of 1 mL/min, and UV detection at 254 nm. Results showed that a good linearity can be found in the range of 0.5~198 μg/mL（r=0.999 98）. The average recovery was 96.40% (n=9). This method was more accurate and sensitive, and can be used to control the chlorhexidine acetate in uterus perfusion agent. |
| Key words: HPLC chlorhexidine acetate content determination |
