| 摘要: |
| 经药敏试验测定,复方苍术口服液和复方十大功劳颗粒对5株耐药大肠杆菌无抑菌活性。但将其加入耐恩诺沙星大肠杆菌的肉汤培养基中进行传代,连传7代后,发现3株菌株的MIC降低了8倍以上,耐药逆转率为60%。其中2号菌株GyrA基因83位氨基酸突变为正常的氨基酸序列,3号菌株ParC基因80位氨基酸也有原来的氟喹诺酮耐药性突变状态转变为正常状态。结果表明中药制剂能逆转大肠杆菌的耐药性。 |
| 关键词: 中药 大肠杆菌 耐药 逆转 |
| DOI： |
| 投稿时间：2012-08-02修订日期：2012-08-02 |
| 基金项目: |
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| Study on the reversal effect of the E.Coli’s resistanceby Chinese traditional medicine |
|
| (China Institute of Veterinary Drug Control) |
| Abstract: |
| Compound Atractylodes oral solution and Compound Mahoniae folium particle haven’t antibacterial activity against 5 resistant E.Coli. analyzed by drug sensitivity test, But the minimum inhibitory concentration(MIC)of 3 strains lower more than 8-fold after the resistant bacteria to enrofloxacin) is sub-cultured continuously over 7 generations in Broth medium with Chinese traditional medicine respectively and reverse ratio is 60%. Ser 83 in GyrA of No.2 strains change into normal series, and Ser 80 in ParC of No.3 strains recover to normal status. The result indicates that Chinese traditional medicine can reverse the resistance of E.coli. |
| Key words: Chinese Traditional medicine E.coli resistance reversal |
