| 摘要: |
| 用犬细小病毒DD株免疫健康易感比格犬，无菌采血后分离血清制备犬抗CPV-2血清。通过SN、ELISA、IFA、HI 方法对制备的血清进行检测，未检出犬类常见的5种病毒抗体，说明该血清特异性良好。经测定，该血清中和抗体效价为1:512；HI效价为1:1280。应用结果表明，该血清对不同犬细小病毒毒株中和能力相当；对细小病毒感染犬有良好的治疗效果。 |
| 关键词: 犬细小病毒 抗血清 制备 应用 |
| DOI： |
| 投稿时间：2014-06-12修订日期：2014-08-01 |
| 基金项目:科技部科技基础性工作专项（2008FY130100） |
|
| Preparation and Application of Canine Anti-Canine Parvovirus Polyclonal Antibody |
|
| (china institute of veterinary drug control) |
| Abstract: |
| Sensitive canines were immunized with CPV DD strain for preparation anti-PCV-2 polyclonal antibody. The collected canine antisera were free of 5 kinds of foreign antibody by SN, ELISA，IFA or HI．The titers of the serum were 1:512 by SN, 1:1280 by HI. The studies showed that the polyclonal antibody had the same neutralizing capality for different CPV-2 strains，and also may be used for treatment of CPV canine. |
| Key words: Canine Parvovirus Polyclonal Antibody Preparation Application |
