| 摘要: |
| 为了建立HPLC法测定麻黄鱼腥草散中黄芩苷、穿心莲内酯、脱水穿心莲内酯含量的方法，采用色谱柱Waters XBrigeTMC18（4.6 mm×150 mm，5μm），黄芩苷以甲醇-0.2%磷酸溶液为流动相梯度洗脱，流速为1.0 mL/min，柱温: 30 ℃；穿心莲内酯、脱水穿心莲内酯以甲醇-水（50∶50）为流动相，流速:1.0 mL/min，柱温为30 ℃；黄芩苷、穿心莲内酯、脱水穿心莲内酯检测波长分别为276、225、254 nm。黄芩苷的线性范围为0.0798~0.7975 μg（r=1.0000），平均回收率为94.3%(RSD=0.8%)。穿心莲内酯的线性范围为0.0109~0.2175μg(r=1.0000)，平均回收率为92.9%(RSD=0.7%)；脱水穿心莲内酯的线性范围为0.0105~0.2105μg(r=0.9999)，平均回收率为92.4%(RSD=0.3%)。本方法快速、简便、准确，能有效测定麻黄鱼腥草散中穿心莲内酯、脱水穿心莲内酯的含量。 |
| 关键词: 麻黄鱼腥草散 黄芩苷 穿心莲内酯 脱水穿心莲内酯 含量测定 HPLC |
| DOI： |
| 投稿时间：2016-08-02修订日期：2016-09-28 |
| 基金项目: |
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| HPLC Determination of Baicalin, Andrographolide and Dehyandrographolide in Mahuang Yuxingcao Powder |
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| (Henan Institute of Veterinary Drug Control) |
| Abstract: |
| A HPLC method had been established to determine the contents of baicalin, andrographolide and dehyandrographolide in Mahuang Yuxingcao powder. The baicalin was analyzed on an XBrigeTM C18 column (4.6 mm×250 mm，5 μm) using ultrapure methanol –0.2% phosphoric acid solution as the mobile phase. The flow rate was 1.0 mL/min. The column temperature was 30 ℃.The andrographolide and dehyandrographolide were analyzed on an XBrigeTM C18 column (4.6 mm×250 mm，5 μm) using methanol –ultrapure water(50∶50) as the mobile phase. The flow rate was 1.0 mL/min. The column temperature was 30℃. The detection wavelengths of Baicalin was set at 276 nm. The calibration curve was linear in the ranges of 0.0798~0.7975 μg (R=1.0000) for baicalin. The recovery of baicalin was 94.3% with RSD of 0.8%. Andrographolide and dehyandrographolide were detected at 225 and 254 nm. The calibration curves were linear in the ranges of 0.0109~0.2175 μg (R=1.0000) and 0.0105~0.2105 μg (0.9999) for andrographolide and dehyandrographolide. The average recoveries were 92.9% for andrographolide (RSD=0.7%, n=6) and 92.4% for dehyandrographolide (RSD=0.3%, n=6), respectively. This method is rapid, simple and accurate，which can be used for the determination of baicalin, andrographolide and dehyandrographolide in Mahuang Yuxingcao powder. |
| Key words: Mahuang Yuxingcao powder baicalin andrographolide dehyandrographolide determination HPLC |
