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| 禽脑脊髓炎、鸡痘二联活疫苗安全性、最小免疫剂量及免疫效力试验 |
| 王红,曹志,毛娅卿,吴涛,宋新宇,宫晓,张恒,马爽,李慧姣,范根成 |
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| (青岛易邦生物工程有限公司 动物基因工程疫苗国家重点实验室) |
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| 摘要: |
| 为确定禽脑脊髓炎(Avian Encephalomyelitis, AE)、鸡痘(Avian Pox, AP)二联活疫苗的安全性、最小免疫剂量及免疫效力。分别检测了3批自制禽脑脊髓炎、鸡痘二联活疫苗的安全性和最小免疫剂量,并对疫苗进行病毒含量测定和攻毒保护试验。结果显示，鸡接种10倍剂量疫苗后精神、食欲及发育均正常，临床表现和剖检均无异常；AEV YBF02株的最小免疫剂量为100 EID50,鸡痘鹌鹑化弱毒株的最小免疫剂量为10 EID50；3批疫苗AEV YBF02株的病毒含量均≥103.2 EID50/羽份,鸡痘鹌鹑化弱毒株的病毒含量均≥103.4 EID50/羽份,攻毒后保护指数为89～100。试验结果表明，自制二联活疫苗安全、有效、质量可控，可用于预防禽脑脊髓炎和鸡痘。 |
| 关键词: 禽脑脊髓炎 鸡痘 安全性 最小免疫剂量 免疫效力 |
| DOI： |
| 投稿时间：2017-10-20修订日期：2018-04-20 |
| 基金项目: |
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| Safety and Minimum Immune Dose and Immune Efficacy Tests of the Combined Avian Encephalomyelitis and Avian Pox Live Vaccine |
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| (Yebio Bioengineering Co,Ltd,Qingdao,Shandong,State Key Laboratory of Animal Genetic Engineering Vaccine) |
| Abstract: |
| The safety，minimum immune dose，virus content and challenge-protection tests were conducted with three batches of the combined Avian Encephalomyelitis（AE）and Avian Pox （AP）live vaccine. The results showed that the spirit，appetite and growth level of the chickens inoculated with tenfold-dose vaccine were the same as those in control post inoculation，there was not any abnormal signs during clinical and autopsy examination；the minimum immune dose of the AEV（Strain YBF02）and the APV（Strain Quail-Adapted）were respectively 100 EID50 and 10 EID50；the virus content of the AEV（Strain YBF02） and APV（Strain Quail-Adapted）were respectively ≥103.2 EID50 /bird and ≥103.4 EID50 /bird；the protective index after challenging was 89～100. These results demonstrated that the safe，stable and effective vaccine is able to prevent poultry against AE and AP. |
| Key words: Avian Encephalomyelitis Avian Pox safety minimum immune dose immune efficacy |
