| 摘要: |
| 随着犬细小病毒变异亚型毒株的增多及所呈现的复杂的流行态势，提高犬细小病毒（CPV）检测方法的实用性、敏感性、特异性和简单快捷性迫在眉睫。通过对传统分子生物学和免疫学检测方法的优化改进，相继衍生出了MT-PCR、iiPCR、IC-LAMP、CPV-GIA、PCR-RFLP和RPA等新型方法，明显提高了检测的时效性及便捷性。就衍生出的多种新型检测方法优势特点进行了综述，以期为CPV的临床和实验室快速检测提供参考。 |
| 关键词: 犬细小病毒 检测方法 分子生物学 免疫学 |
| DOI： |
| 投稿时间：2018-04-18修订日期：2018-07-19 |
| 基金项目:十三五国家重点研发项目（2017YFD0501703, 2016YFD0501002） |
|
| Progress of Diagnostic Methods for Canine Parvovirus Disease |
|
| (College of Animal Science and Technology,Jilin Agricultural University Changchun 130118) |
| Abstract: |
| With the increase of the variant subtypes of canine parvovirus (CPV) and the complicated epidemic situations, it is imminent to improve the practicability, sensitivity, specificity and simplicity of the detection methods of CPV. By optimizing the traditional molecular biology and immunological detection methods, new methods such as MT-PCR, iiPCR, IC-LAMP, CPV-GIA, PCR-RFLP and RPA have been consecutively derived, which improve the timeliness and convenience of the detection obviously. The advantages and characteristics of various new detection methods were summarized in order to provide an authentic reference for clinical and laboratory rapid detection of CPV. |
| Key words: canine parvovirus detection methods molecular biology immunology |
