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鸡胚母源抗体对新城疫Lasota毒株繁殖的干扰及克服方法
王瑞,徐太辉
0
(吉林大学农学部动物医学专业;青岛易邦生物工程有限公司)
摘要:
鸡胚中过高的母源抗体会干扰Lasota毒株的繁殖。本试验结果证明,当母源抗体>26时种毒(EID50 108.5,HA 210)稀释1×103-5×103倍;母源抗体平均24左右时,种毒稀释5×103-1×104倍;母源抗体降至23左右时,种毒稀释倍数1×104-1×105为宜。按以上方法接种鸡胚,所收毒液效价最高。
关键词:  新城疫病毒  母源抗体  病毒繁殖  种毒稀释  效价
DOI:
投稿时间:2007-11-05修订日期:2007-12-14
基金项目:
The Maternal Antibody in Chick Embryo Influence Newcastle DiseaseVirus Lasota Strain to Reproduce and Method to Reduce the Influence
wang rui,徐太辉
(griculyural college Jilin university)
Abstract:
High maternal antibody in chick embryo influenced reproduction of Newcastle Disease Virus(Lasota strain).The result of experiment showed when the maternal antibody was over26(HI),The seed virus(EID50 108.5, HA 210)was diluted 1×103-5×103times, when the maternal antibody was 24,The seed virus was diluted5×103-1×104times, when the maternal antibody was 23, The seed virus was diluted 1×104-10×104times, According to this method to inoculate chick embryo ,The titer of virus in embryo water was the highest.
Key words:  Newcastle Disease Virus  Maternal antibody  Breed reproducte  Dilute,  Titer.

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