| 摘要: |
| 以金黄色葡萄球菌为试验菌，抗生素检定培养基Ⅲ号为试验用培养基，将泰乐菌素稀释成不同的浓度，寻找浓度的对数值与相对应的吸光度呈直线相关的线性范围，建立测定泰乐菌素及其制剂含量的浊度法。泰乐菌素的浓度在0.6 u/ml～2.0 u/ml之间，其浓度的对数值与相对应的吸光度呈直线相关，相关系数：r＝0.9992。经t检验，泰乐菌素浓度的对数值与相对应的吸光度回归效果显著。所建立的浊度法平均回收率为99.87％，平均可信限率为2.76％，RSD为0.37％。管碟法与浊度法所测结果无显著差异。浊度法具有简便、精确、快速的特点，可用泰乐菌素及其制剂含量的测定。 |
| 关键词: 浊度法 管碟法：泰乐菌素 |
| DOI： |
| 投稿时间：2007-12-19修订日期：2008-02-26 |
| 基金项目: |
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| Determination of Tylosin Potency by Turbidimetric Method |
| banfuguo |
| (Henan Institute of Veterinary Drug Control) |
| Abstract: |
| For developing turbidimetric method to determine tylosin and tylosin tartrate soluble power, tylosin was diluted to different concentrations to find the linear correlation range of log values of concentrations and absorbabilities by using staphylococcus aureus as test organism and antibiotics standardization medium III as test medium. At the concentration range from 0.6 u / ml to 2.0 u / ml，log values of concentration of tylosin were linear correlation with absorption values, which correlation coefficient was r = 0.9992. By the t-test, regression effect of log values of concentrations of tylosin with absorbabilities of cultures was significant. Average recovery is 99.87％，and average confidence rate is 2.76％，and relative standard deviation is 0.37％. Potencies were unitied which determined by turbidimetric method and cylinder-plate method. Turbidimetric method is simple, accurate and fast,so that can be used to determine tylosin and tylosin tartrate soluble power. |
| Key words: turbidimetric method cylinder-plate method tylosin |
