| 摘要: |
| 目的 薄层扫描法测定比较三种不同皂苷提取方法对黄芪甲苷含量的影响。方法 分别采用大孔树脂吸附法、正丁醇萃取法和KOH回流提取法制备供试品。以氯仿-甲醇-水为展开系统，薄层扫描法测定黄芪甲苷含量。结果 大孔树脂吸附法、正丁醇萃取法和KOH回流提取法三种方法提取物中黄芪甲苷平均含量分别为0.07768%，0.05578%，0.06953%。结论 大孔树脂法提取黄芪所得黄芪甲苷含量较高。 |
| 关键词: 黄芪皂苷 提取 黄芪甲苷 含量测定 薄层扫描法 |
| DOI： |
| 投稿时间：2008-01-11修订日期：2008-02-23 |
| 基金项目:国家自然科学基金项目（30600821） |
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| Effects of Three Saponin Extracting Methods on Content of Astragaloside IV |
| XUXIANXIANG |
| (China Pharmceutical University) |
| Abstract: |
| Objective To determine content of astragaloside IV by TLC-scanning in three extraction with different methods. Methods Three methods including D101 macroreticular resin method, extraction refine by n-butyl alcohol and KOH-regurgitate pheresis were applied to Astragalus saponins extracting, contents of astragaloside IV were determined by TLC-scanning. The mobile phase was chloroform-methanol-water. Results The average contents of Astragaloside IV was 0.07768% by D101 macroreticular resin method, 0.05578% by extraction refine by n-butyl alcohol and 0.06953% by KOH-regurgitate pheresis. Conclusions The method of macroreticular resin is better than two others. |
| Key words: Astragalus saponins extracting Astragaloside IV content determination TLC-scanning |
