| 摘要: |
| 建立了鸡肉组织中甲硝唑、洛达硝唑、地美硝唑、替硝唑、塞克硝唑、奥硝唑6种硝基咪唑类药物及地美硝唑代谢产物1-甲基-5-硝基-2-羟甲基咪唑（HMMNI）共 7种组分残留检测的高效液相色谱（HPLC）法。样品采用乙酸乙酯提取，SCX柱净化；色谱条件：色谱柱为Eclipse Plus C18柱 (4.6×150mm,3.5µm)；流动相为水-乙腈-甲醇，梯度洗脱；柱温为35℃；流速为0.8mL/min，紫外检测波长为320nm，外标法定量。结果表明：7种组分在5-60ng/mL范围内，呈良好线性关系，相关系数R2均>0.998；方法检出限为 1µg/kg；添加浓度分别为2、3、5µg/kg时，平均回收率为66.6%~101%，批内批间RSD均小于20%。 |
| 关键词: 硝基咪唑类药物 代谢物 残留 HPLC 鸡肉 |
| DOI： |
| 投稿时间：2008-06-12修订日期：2008-06-12 |
| 基金项目: |
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| Simultaneous Determination of Seven Nitroimidazole Residues in Chicken by HPLC |
| huhaiyan |
| (China Institute of Veterinary Drug Control, Beijing) |
| Abstract: |
| A HPLC method was established for the seven nitroimidazole including metronidazole,ronidazole,tinidazole,secnidazole,ornidazole,dimetridazole and 2-hydroxymethyl-1-methyl-5-nitroimidazole（HMMNI）.The sample was extracted with ethyl acetate，and then was purified by strong cation exchange（SCX） solid phase extraction cartridge；HPLC conditions：the chromatography column was Eclipse Plus C18 column of 4.6×150mm，3.5µm；mobile phase was water-acetonitrile -methanol；column temperature was 35℃；flow rate was 0.8mL/min； Determination was by UV detection at 320nm. The calibration curve was good linear between the peak areas and concentrations of nitroimidazole from 5 to 60ng/mL,the correlation coefficient R2>0.998. The limit of detection was 1µg/kg； The average recoveries from spiked chicken at the three concentrations of 2，3 and 5 µg/kg ranged from 66.6%~101%，and intra- day and inter-day（n=5） batch variation coefficients were less than 20%. |
| Key words: nitroimidazole drugs metabolites residue HPLC chicken |
