| 摘要: |
| 以大肠杆菌为试验菌，抗生素检定培养基Ⅲ号为试验用培养基，研究比浊法测定硫酸黏菌素及其制剂的含量。硫酸黏菌素的浓度在44.9 u/ml～93.2u/ml之间时，检测菌液接种浓度为0.3%，培养时间为2～3h，其浓度的对数值与相对应的吸光度具有良好的线性关系，相关系数：r＝0.9978。所建立的浊度法重现性较好,具有简便、精确、快速的特点，可用于硫酸黏菌素及其制剂含量的测定。 |
| 关键词: 浊度法 硫酸黏菌素 含量测定 |
| DOI： |
| 投稿时间：2008-07-23修订日期：2008-08-18 |
| 基金项目: |
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| Determination of the Potency of Polymyxin by Turbidimetric Method |
| Ban Fuguo,高延玲 |
| (Henan Institute of Veterinary Drug Control, Zhengzhou, Henan, 450008,China) |
| Abstract: |
| Abstract: A turbidimetry assay for fast determination of colistin sulfate was developed. E.coli as test organism and antibiotics standardization medium Ⅲ as test medium were used.There was a linear relationship between the potency of colistin sulfate and the logarithm of absorbency in the range of 44.9 u/ml～93.2u/ml with inoculating concentration of 0.3% and culturing time of 2～3h. The turbidimetry assay is accurate and fast,it could be used to determine colistin sulfate .
Key words: turbidimetric method；colistin sulfate；determination of the potency
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| Key words: turbidimetric method polymyxin E |
