| 摘要: |
| 建立了超高效液相色谱-串联四级杆质谱法测定原料乳中苯并咪唑类药物残留量的检测方法。原料乳经乙酸乙酯提取,MCX固相萃取柱净化后用UPLC-MS/MS进行检测。色谱条件: ACQUITY UPLCTM BEH C18柱（2.1mm×100mm i.d.,1.7μm）；流动相：乙腈-0.1%甲酸溶液,梯度洗脱，流速0.3 mL/min。采用正离子模式检测,外标法定量。本方法检测测苯并咪唑类药物残留的检测限为0.5μg /kg，定量限为1.0μg /kg。 |
| 关键词: 苯并咪唑类药物 超高效液相色谱-串联四级杆质谱 原料乳 |
| DOI： |
| 投稿时间：2009-02-01修订日期：2009-03-23 |
| 基金项目: |
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| Determination of benzimidazoles residue in raw milk using ultra performance liquid chromatography-tandem mass spectrometry |
| fang zhongyi |
| (Henan provincial supervising agency of veterinary Drugs) |
| Abstract: |
| Abstract:A method for determination of benzimidazoles residue in raw milk was developed using ultra performance liquid chromatography- tandem mass spectrometry (UPLC -MS/MS). Ethyl acetate was used to extract the target analyte from raw milk，extraction was degreased and cleaned up with Waters Oasis MCX solid phase extraction (SPE) column for UPLC- MS/MS. The liquid chromatography separation was carried on C18 column（2.1mm×100mm i.d.,1.7μm）with acetonitrile and water(added 0.1% formic acid) as mobile phase at a flow rate of 0.3mL/min.The mass spectrometry was performed in multiple reaction monitoring(MRM)mode. The limits of detection (LOD) of the method were 0.5μg/kg and the limits of quantification (LOQ) were 1.0μg/kg. The method has good repeatability and high sensitivity ,and can be applied for the determination of benzimidazoles in raw milk. |
| Key words: benzimidazoles ultra performance liquid chromatography-tandem mass spectrometry raw milk |
