| 摘要: |
| 利用高效液相色谱法测定博落回注射液中血根碱和白屈菜红碱的含量。采用Agilent1100型高效液相色谱仪，色谱柱为Agilent Ecplise XDB－C18（5μm,4.6×250mm），流动相为三乙胺溶液－乙腈（75：25，V/V），流速：1.0mL/min；检测波长：270nm；柱温：35℃。结果表明：血根碱在0.5～100μg/mL范围内线性关系良好（R2＝0.9999）,平均加样回收率（n=3）为96.1％～97.9％，RSD为0.8％～1.8％；白屈菜红碱在0.5～100μg/mL范围内线性关系良好（R2＝1），平均加样回收率（n=3）为96.8％～98.8％，RSD为0.6％～1.0％。本法简单快捷，精密度好，可为制定质控标准提供参考。 |
| 关键词: 高效液相色谱法，博落回注射液，血根碱，白屈菜红碱，含量 |
| DOI： |
| 投稿时间：2009-02-06修订日期：2009-03-04 |
| 基金项目: |
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| Simutaneous Determination of Sanguinarine,Chelerythrine in Macleaya Cordata Injection by HPLC |
| XU Shi-fu |
| (Anhui Provincial Control Institute of Veterinary Pharmaceuticals and Feed) |
| Abstract: |
| To develop an HPLC mothod for determination of Sanguinarine,Chelerythrine in
Macleaya Cordata injection.By Agilent 1100 highly effective liquid phase chromatograph, the Agilent Ecplise XDB-C18 (5μm, 4.6×250mm)was used as analytical column with a mobile phase consisted of triethylamine solution - acetronitrile (75:25，V/V).The flow rate was 1.0mL/min with ulteaviolet detection wavelength at 270nm and column temperature was 35℃.Finally the founded method linearity of sanguinarin was in the range of 0.5~100μg/mL, (R2=0.9999), The average recovery rate (n=3) was 96.1%~97.9%, RSD was 0.8%~1.8%; And the founded method linearity of Chelerythrine was in the range of 0.5~100μg/mL, (R2=1), The average recovery rate (n=3) was 96.8%~98.8%, RSD was 0.6%~1.0%; This method is simple ,quickly ,sensitive and can be used as a reference for the standard of quality control
.
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| Key words: HPLC Macleaya Cordata Injection sanguinarine chelerythrine Simutaneous |
