| 摘要: |
| 对磺胺喹噁啉钠采用硅胶GF254板为固定相，以正丁醇－浓氨水（15：3）为展开剂，用薄层色谱鉴别中兽药散剂中磺胺喹噁啉钠分离较好，斑点显色清晰且无干扰，可用于中药散剂中非法添加物磺胺喹噁啉钠的快速筛选。采用C18柱为固定相，以甲醇-乙腈-水-冰乙酸（2：2：9：0.2）为流动相，紫外检测波长为270 nm。液相色谱鉴别中药散剂中非法添加磺胺喹噁啉钠分离良好，无干扰峰，可用于中兽药散剂中非法添加物磺胺喹噁啉钠的鉴别。 |
| 关键词: 薄层色谱法 液相色谱法 磺胺喹噁啉钠 |
| DOI： |
| 投稿时间：2009-10-28修订日期：2010-01-11 |
| 基金项目: |
|
| Identification Sulfaqiunoxline Sodium Adding to Herb Veterinary Drug Compound Preparation by TLC and HPLC |
| gaoyingchun |
| (Shandong Provincial Veterinary Medicine Supervision Institute) |
| Abstract: |
| TLC and HPLC method were established for the determination of sulfaqiunoxline sodium in herb veterinary drug compound preparation. TLC condition: Drug was separated on silica gel thin layer chromatography plate；developing agent was n-butanol/stronger ammonia water(15∶3).HPLC conditons: the chromatography column was C18 column of 150 mm×4.6 mm,5 μm；mobile phase was methanol-acetonitrile-water-acetic acid (2∶2∶9∶0.2,V/V)；Determination was by UV detection at 270 nm. This meathod identifying sulfaqiunoxline sodium adding to herb veterinary drug compound preparation is successfully feasible. |
| Key words: TLC,HPLC, Sulfaqiunoxline sodium |
