| 摘要: |
| 本文建立了HPLC-ELSD法测定黄芪多糖注射液中黄芪甲苷的含量。采用Agilent Ecplise XDB-C18柱（250mm×4.6mm，5µm）；流动相：乙腈—水（35：65）；流速1.0ml/min；柱温30℃；蒸发光散射（ELSD）检测器；漂移管温度：105℃；气体流速：2.70L.min-1，进样量20µL。结果表明：黄芪甲苷在10~400µg.mL-1范围内线性关系良好（R2=0.9998），平均加样回收率（n=3）为98.9%～99.6%，RSD为0.8%~1.8%。结果表明本法简便，快速，准确，重现性好，可用于控制该制剂的质量。 |
| 关键词: 黄芪多糖注射液 黄芪甲苷 HPLC-ELSD |
| DOI： |
| 投稿时间：2010-01-08修订日期：2010-01-30 |
| 基金项目: |
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| Determination of AstragalosideⅣ in Astragalus Polysaccharide injection by HPLC-ELSD |
| liu hong yun |
| (nHui province control institute of Veterinary and feed) |
| Abstract: |
| A high performance Liquid chromatographic- ELSD method was reported for quantitative determination of AstragalosideⅣ with Agilent Ecplise XDB-C18 Column（250mm×4.6mm，5µm）, mobile phsae acetomical—water(35：65); flow rate was 1.0ml/min; column temperature was 30℃;.The temperature of drift tube for measurement was 105℃ and the flow rate of N2 was 2.70L.min-1.On these conditions, the linear ranges were 10~400µg.mL-1(R2=0.9998)The average recovery was 98.9%～99.6% . The method was sensitive , reliable, accurete and repeatability, it can be used for quality control method of Astragalus Polysaccharide injection. |
| Key words: Astragalus Polysaccharideinjection AstragalosideⅣ HPLC-ELSD |
