| 摘要: |
| 对酸性碱性中性等多种单糖进行1-苯基-3-甲基-5-吡唑啉酮(pmp)衍生优化，得到混合单糖pmp衍生的最佳条件为反应时间60min，反应温度70 ℃，衍生试剂PMP与单糖的最适摩尔比为12：1，加入的碱与单糖最适摩尔比为6：1。并在此条件下对水解盐藻多糖进行衍生分析得到盐藻多糖组分PDS3的单糖组成。 |
| 关键词: 多糖 单糖组成 pmp柱前衍生 |
| DOI： |
| 投稿时间：2010-01-11修订日期：2010-01-14 |
| 基金项目: |
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| Monosaccharide Composition Analyses of polysaccharide Veterinary Drug |
| Fu haining |
| (Qingdao Continent Pharmaceutical Group) |
| Abstract: |
| In order to determine the best dirive condition of mixed monosaccharides, we optimized the 1-phenyl-3-methyl-5-pyrazolone dirived condition of three kinds of monosaccharides(uronic acid, aminosaccharide and neutral sacchraide). The best condition we got is as follows: reaction time is 60 min, incubation temperature is 70 ℃, ratio of NaOH and monosaccharide is 6:1, ratio of PMP and nonosaccharide is 12:1. Under this contion we got the monosaccharide composition of PDS3. |
| Key words: polysaccharide Monosaccharide Composition precolumn-derivatization high performance liquid chromatography |
