| 摘要: |
| 运用微波诱变结合消泡剂定向选育VB12生产菌株—脱氮假单胞杆菌。结果表明：当微波功率800W、脉冲频率2450MHz，分离平皿中消泡剂加量3‰时，诱变剂量6s效果最明显。得到遗传稳定的突变菌株VB12Ⅰ09-1023#，通过摇瓶测得该突变株发酵单位高达268mg/L，比出发菌株提高了28.8%。120m3发酵罐试验，25批平均发酵单位高于对照7.5%。 |
| 关键词: 微波诱变 消泡剂 脱氮假单胞杆菌 发酵 |
| DOI： |
| 投稿时间：2010-07-15修订日期：2010-09-27 |
| 基金项目: |
|
| Microwave Induced by Combining Antifoaming agent Directional |
| shenxiaohong |
| (Ningxia Institute of Multi-strain Duowei Tairui Pharmaceutical Co.，Ltd) |
| Abstract: |
| Using microwave induced by combining antifoaming agent directional selection VB12 production pseudomonas denitrificans.The result shows that: when microwave power 2450MHz 800W, pulse frequency, separation AGAR disinfection defoamer quantity 3 per 1,000, mutagenic effect is the most obvious. Dose 6s Get VB12 strains of mutation genetic stability Ⅰ 09-1023 #, measured by the wave bottle fermentation unit as mutations 268mg/L, improved strains of 28.8%. 120m3 fermentation tests, an average of 25 batch fermented unit ck 7.5%. |
| Key words: microwave mutagenesis antifoaming agent nitrogen false aeromonas fermentation |
