| 摘要: |
| 摘要：利用分子生物学软件DNASTAR对含有亲和标记、Xa factor识别序列和连接肽的DAB389(Gly4Ser)2-aMSH以及DAB389-aMSH的表位、亲水性以及等电点等生物学特性进行了预测分析，结果显示DAB389(Gly4Ser)2-aMSH的表位为0.8，DAB389-aMSH为2.1；亲水性为2.7高于DAB389-aMSH的1.96；两者的等电点均为5.9；His×Tag、Xa factor识别序列使重组蛋白的亲水性增强，表位降低；重组蛋白氨基端前70个氨基酸中不存在信号肽；DAB389(Gly4Ser)2-aMSH的表达量低于DAB389-aMSH 2%左右，前缀对蛋白表达量有一定的影响。 |
| 关键词: 前缀 重组蛋白 亲水性 表位 信号肽 |
| DOI： |
| 投稿时间：2010-08-19修订日期：2010-09-05 |
| 基金项目: |
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| Predictive analysis in effect the bionomics of prefixing to recombination protein |
| lizehong |
| (Jilin Agriculture University) |
| Abstract: |
| Abstract: The designed fusion protein DAB389(Gly4Ser)2-aMSH and DAB389-aMSH include affinity labeling, Xa factor, (Gly4Ser)2, was analyzed and compared in some molecular biology characteristics by using molecular biology software DNASTAR. The results showed that the epitope of DAB389(Gly4Ser)2-aMSH was 0.8 lower/less than 2.1 of DAB389-aMSH, it's hydrophilicity was 2.7 higher than 1.96 of DAB389-aMSH, The isoelectric point of them was 5.9. His×Tag, Xa factor recognition sequence made the hydrophilicity strengthen, epitope and antigenicity depress; the pro 70 amino acid in N-terminal of recombination protein had no signal peptide, the expression quantity of DAB389(Gly4Ser)2-aMSH was less 2% than that of DAB389-aMSH, these prefixing had determinate influence to expression protein. |
| Key words: prefix Recombination protein Hydrophilicity Epitope Signal peptide |
