| 摘要: |
| 文章介绍了链置换扩增技术、转录介导扩增技术、核酸序列依赖性扩增技术、环介导等温扩增技术、赖解旋酶恒温基因扩增技术、滚环扩增等六种恒温扩增核酸的技术的特点及其研究应用概况，并对恒温扩增技术的前景予以展望，以期为这些技术的应用提供参考。 |
| 关键词: 恒温扩增 病原检测 展望 |
| DOI： |
| 投稿时间：2010-09-25修订日期：2010-09-25 |
| 基金项目: |
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| Current status and prospects of Isothermal amplification in thedetection pathogens of animal disease |
| liuyebing |
| (China Institute of Veterinary Drug Control) |
| Abstract: |
| In the paper, an overview of the current status relating to Isothermal amplifications including Strand Displacement Amplification, Transcription mediated amplification, Nucleic acid sequence-based amplification, Loop-mediated isothermal amplification, Helicase-dependant amplification and Rolling circle amplification were reviewed, and the features, applications, the challenges and the techniques problems of them were discussed . The prospects and further applications of Isothermal amplifications in their future development were considered,too. |
| Key words: Isothermal amplification Pathogen Detection Prospects |
