| 摘要: |
| 摘要：目的：建立控制复方黄芪金银花颗粒剂的质量标准。方法：采用薄层层析对颗粒中女贞子、金银花、黄芪、枸杞子进行了薄层鉴别；采用双光束紫外-可见光分光度法测定了颗粒中绿原酸的含量。结果：薄层鉴别方法专属性强，含量测定绿原酸在42.24—84.48ug /ml内具有良好的线性关系,标准曲线回归方程为：A=0.0053證－0.0012（r=0.9999，n=5）；样品在24h内稳定，RSD=0.733%；加样平均回收率为102.049%，RSD=0.499%；该方法精密度高，RSD=0.382%。结论：本质量标准可有效地控制复方黄金颗粒剂的质量。 |
| 关键词: 复方黄芪金银花颗粒剂 薄层色谱法 紫外分光光度法 绿原酸 |
| DOI： |
| 投稿时间：2011-04-11修订日期：2011-05-13 |
| 基金项目: |
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| Study the standard of Huangjin Compound granules |
| zhaishaoqin |
| (Chongqing Academy of Animal Science) |
| Abstract: |
| Abstract: Objective: Establish control standards of Huangjin compound granules.Methods: Using the Thin layer chromatography to distinguish Astragalus, Lonicera japonica, Ligustrum lucidum, Chinese wolfberry fruit, etc. in the particles. Dual-beam UV - visible spectrophotometry in the determination of the particle content of chlorogenic acid. Results: TLC method is specific. Determination of chlorogenic acid has good linearity within the the 42.24-84.48ug / ml .The Standard curve regression equation: A=0.0053證－0.0008（r=0.9999，n=5）. The Sample is stable in the 24h, RSD=0.733%. The average recovery is 102.049%, RSD=0.499%. This method was high Precision, RSD=0.382%.Conclusion: The quality standards that can effectively control the quality of Huangjin compound granules. |
| Key words: Huangjin compound granules ,TLC, Dual-beam UV-visible spectrophotometry, Chlorogenic acid |
