| 摘要: |
| 本研究选用pNZ8149-COE NZ3900重组乳酸菌作为绿色荧光蛋白(green fluorescent protein，GFP)的表达载体，制成活菌制剂，口服免疫小鼠，以GFP基因作为标记跟踪重组菌在小鼠体内的定位，并利用荧光定量PCR技术对各部位菌定量分析。结果表明，由本研究构建的重组乳酸乳球菌PEDV-COE-GFP NZ3900可较长时间定植于胃肠黏膜表面，为基因工程重组乳酸菌口服疫苗的研究奠定了基础。 |
| 关键词: 猪流行性腹泻病毒 乳酸菌 绿色荧光蛋白 定植 荧光定量PCR |
| DOI： |
| 投稿时间：2011-04-24修订日期：2011-06-03 |
| 基金项目:吉林省教育厅基金2010322；吉林农业大学青年启动基金2009083 |
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| Studies on colonization of recombine Lactococcus lactis with PEDV COE gene in mice |
| dong hao |
| (Jilin Agricultural University) |
| Abstract: |
| For this research, pNZ8149-COE were chosen as the expression vector of GFP(green fluorescent Protein).The BALB/c mice were orally dosed with approximately GFP-labeled Lactococcus lactis NZ3900.The lactis in the body of the mice was detected by FQ-PCR. The results show that the recombined lactic acid bacteria PEDV-COE NZ3900 could field planting gastrointestinal tract for longer time, which has provided a ground for research of genetic engineering recombined lactic acid bacteria oral vaccine. |
| Key words: PEDV lactic acid bacteria GFP colonizati FQ-PCR |
