| 摘要: |
| 目的:建立快速、稳定的家兔血浆中地克珠利的提取方法，为进一步研究家兔组织中地克珠利的残留量提供基础。方法：采用HPLC法以妥曲珠利为内标，N,N-2甲基甲酰胺及乙腈联合沉淀法提取家兔血浆中地克珠利，色谱柱为Hypersil ODS （2.5 μm，250 mm?4.6mm），流动相为乙腈:水:三乙胺:冰乙酸＝55:45:0.2:0.12(v/v)，流速为1.0ml/min，紫外检测波长为240 nm。结果：在0.156~20.0 μg/mL浓度范围内，地克珠利的峰面积与其浓度呈现良好的线性关系（r2＝0.9992）；回收率为98.9%～102.4％；方法精密度RSD最大值为6.8％；最低检测限为0.078 μg/mL。结论：DMF及乙腈联合沉淀法适用于家兔血浆中的地克珠利的提取。 |
| 关键词: 家兔 血浆 地克珠利 HPLC |
| DOI： |
| 投稿时间：2011-05-09修订日期：2011-06-14 |
| 基金项目:现代农业产业技术体系建设专项资金（nycytx-44-2-4） |
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| HPLC method for the determination of diclazuril in rabbit plasma |
| yangliping |
| (Institute of Animal Science and Veterinary Medicine,) |
| Abstract: |
| Objective: To establish a rapid and stable tissue extraction method for the determination of diclazuril residues in rabbit plasma. Methods: Diclazuril and Toltrazuril（internal standard）were added in blank rabbit plasma, extracted by N,N-dimethylformamide (DMF)/acetonitrile precipitation method and determined by HPLC(Hypersil ODS-C18).The mobile phase consisted of a mixture of tirethylamine/water/triethylamine/aceticacid (55:45:0.2:0.12) at the flow rate was 1.0ml?min-1;The ultraviolet detection wavelength was set at 240nm.. Results: The method was proven to be linear over a plasma diclazuril concentration range of 0.156 to 20.0 μg/mL with a mean correlation coefficient of 0.9992; The recovery of diclazuril from rabbit plasma was found to be 98.9～102.4％; The max RSD of method precision was found to be 6.8%; The detection limit was 0.078 μg/mL Conclusions: DMF/acetonitrile precipitation method is suitable for the extraction of diclazuril in rabbit plasma. |
| Key words: Rabbit plasma Diclazuril HPLC |
