| 摘要: |
| 通过研究不同类型培养基和培养温度、起始pH值等发酵条件对hIFN-γ产量的影响,初步确认了摇瓶水平发酵各因素的最优条件，优化的发酵条件为发酵起始pH值7.0、培养温度37℃、IPTG诱导浓度1.0mmol/L、菌体生长密度OD600达到1.0时加入IPTG、诱导时间为3h。此时hIFN-γ产量达到最高水平，从而为hIFN-γ批量生产奠定了良好的基础。 |
| 关键词: 重组人γ-干扰素，摇瓶发酵，表达 |
| DOI： |
| 投稿时间：2011-05-26修订日期：2011-06-22 |
| 基金项目:国家自然科学基金项目（面上项目，重点项目，重大项目） |
|
| Optimization of Fermentation Condition of Recombinant Strain BL21(DE3)(pET32a( )-hIFN-γ) in Shaking Flask |
| xuchongli |
| (College of Environment and Bioengineering, Jilin Institute of Chemical Technology) |
| Abstract: |
| The expression level of hIFN-γ was influenced by the different medium and other fermentation conditions including culture temperature, initial pH and so on.Various factors optimal conditions was confirmed by flask- fermentation.The optimal fermentation condition with IPTG induction is culture medium pH7.0,culture temperature 37℃, joining IPTG to final concentration 1.0mmol/L when the recombinant strain growth density OD600 achieved 1.0, and induction time 3h. The production of hIFN-γ reached the highest level.This fermentation technology established a good foundation for the hIFN-γmass production. |
| Key words: Recombinant human Interferon-γ,flask- fermentation,expression |
