| 摘要: |
| 建立一种简单、实用的鸡蛋中苏丹红Ⅰ、Ⅱ、Ⅲ、Ⅳ残留的检测方法。样品用乙腈∶丙酮= 9∶1(V∶V)溶液提取，经凝胶渗透色谱（GPC）净化后，反相液相色谱分离测定。本方法的检测限为20μg/kg，定量限为50μg/kg。四种苏丹红染料在20～2000ng/mL范围内呈线性相关。在空白鸡蛋中添加50～1000礸/kg浓度下，四种苏丹红的平均回收率为65%～85％，批内变异系数在1.0～4.8％之间（n＝5），批间变异系数在2.1～5.9％之间（n＝3）。结果表明，该法简单、灵敏、特异性强、重现性好，适用于鸡蛋中苏丹红Ⅰ、Ⅱ、Ⅲ、Ⅳ残留的测定。 |
| 关键词: 苏丹红 鸡蛋 残留 凝胶渗透色谱 超高效液相色谱 |
| DOI： |
| 投稿时间：2011-06-02修订日期：2011-07-22 |
| 基金项目: |
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| Determination of Sudans Residues in Egg by Gel Permeation Chromatography-ultra performance liquid chromatography |
| wuningpeng |
| (Henan Institute of Veterinary Drug Control) |
| Abstract: |
| Gel Permeation Chromatography-ultra-performance liquid chromatography method for simultaneous determination of sudanⅠ、Ⅱ、Ⅲ and Ⅳ was developed.The sampkes were extracted by acetonitrile: aceton(9:1), cleaned up by Gel Permeation Chromatography, and deteminated by ultra-performance liquid chromatography. The limit of detection was 20μg/kg. The limit of quantification was 50μg/kg .The calibration curves of the four sudan dyes were linear over the concentration ranges of 20～2000ng/mL.The average recoveries ranged from 65% to85%.The inter-run relative standard deviation(RSD) was in the range of 1.0～4.8％(n=5),and the between-run relative standard deviation(RSD) wasin the range of 2.1～5.9% （n＝3）. Because of its high sensitive,good precision and specificity,the method was found to be suitable for trace analysis of sudans in eggs. |
| Key words: sudans egg residues GPC UPLC |
