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进口动物产品中莱克多巴胺残留的快速检测
陈茹
0
(广东出入境检验检疫局技术中心)
摘要:
应用经验证可靠的酶联免疫吸附试验(ELISA)方法,对进口猪产品进行莱克多巴胺残留快速筛选检测,对ELISA检测阳性的样品采用色谱分析方法进行确证检测。对1330份猪产品的检测结果显示,采用ELISA方法检出阳性率达11.4 %(152/1330),其中采用高效液相色谱-串联质谱(HPLC-MS/MS)或气相色谱-质谱方法(GC-MS)确证阳性率达90.8 % (138/152)。检测工作表明,综合运用快速筛选检验技术与确证检测技术可实现快速准确检测动物产品中莱克多巴胺残留,提高进出境检验工作效率。
关键词:  莱克多巴胺  动物产品  ELISA  高效液相色谱-串联质谱  气相色谱-质谱  
DOI:
投稿时间:2011-06-09修订日期:2011-06-27
基金项目:
Rapid detection of Ractopamine residues in imported animal products
CHEN RU
(Technical centre of Guangdong Entry-exit Inspection and Qurantine Bureau)
Abstract:
A validated ELISA method was applied to screening detection of ractopamine residues on imported porcine products, and positive samples were further determined by chromatography confirmatory analysis method. The ELISA detected 11.4 % (152/1330) positive from a total of 1330 porcine raw products, and 90.8% (138/152) samples were confirmed as positive by HPLC-MS/MS or GC-MS methods. The combined application of rapid screening method and confirmation method could achieve rapid and accurate detection of ractopamine residues on animal products and help improve the import and export inspection practices.
Key words:  Ractopamine, animal products, ELISA, HPLC-MS/MS, GC-MS/MS,

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