| 摘要: |
| 研究不同类型培养基、诱导时间、菌体密度和不同补料对hIFN-γ表达的影响,并运用高密度发酵工艺对hIFN-γ的产量进行优化，目的蛋白表达量从4000mg/L提高到20000mg/L以上，发酵产量提高了5倍，大大提高了效率。同时为验证工艺的稳定性，将优化的发酵条件进行连续三批重复发酵实验。结果表明该优化条件具有重复性,表达稳定，维持在40%左右，从而为hIFN-γ大规模生产奠定了良好的基础。 |
| 关键词: 重组人γ-干扰素 发酵 优化 |
| DOI： |
| 投稿时间：2011-06-13修订日期：2011-09-29 |
| 基金项目:国家自然科学基金项目（30972188） |
|
| Optimization of Fermentation Technology of Recombinant Human Interferon-γ |
| xuchongli |
| (College of Environment and Bioengineering, Jilin Institute of Chemical Technology) |
| Abstract: |
| The expression level of hIFN-γ was influenced by the different medium, induction time, cell density and feeding batch. The production of hIFN-γ was optimized by high density fermentation. The expression level of hIFN-γ was increased from 4000mg/L to over 20000mg/L, the fermentation yield was increased by 5 times. In order to confirm the technical stability, we performed a three-time repeated fermentation. The result indicated that the optimal conditions had the repeatability and the expression level remained 40% or so. This fermentation technology established a good foundation for the hIFN-γ mass production. |
| Key words: Recombinant human Interferon-γ fermentation optimization |
