| 摘要: |
| 为进一步优化猪细小病毒HA、HI微量法试验，比较了不同红细胞悬液浓度、不同液体体积、不同形状微量板对试验的影响。结果表明，豚鼠红细胞悬液浓度由原来的0.25%提高到0.5%，用50 μL体积进行HA试验，用25 μL体积进行HI试验，在“V”型微量板上的试验结果更清晰、更易判断。 |
| 关键词: 猪细小病毒 HA试验 HI试验 微量板 |
| DOI： |
| 投稿时间：2011-06-20修订日期：2011-08-18 |
| 基金项目: |
|
| Optimization of HA and HI Micro-test for the Porcine Parvovirus |
| 张婉华 |
| (Shanghai Jiamu biological products Co. Ltd.) |
| Abstract: |
| The concentrations of red blood cells, reaction solution volume and microplate bottom shape were optimized for porcine parvovirus (PPV) HA and HI test. Compared with the microplate of U-shape bottom, HA and HI test for PPV on the microplate of V- shape bottom was more definite and convenient to read the results with increased guinea pig’s RBC concentration from 0.25% to 0.5%, 50 μL reaction solution for HA test and 25 μL reaction solution for HI test. |
| Key words: porcine parvovirus HA test HI test microplate |
