| 摘要: |
| 了解上海地区规模化猪场分离的大肠埃希菌产超广谱β-内酰胺酶(ESBLs)的分离率、耐药性和基因型。收集2010年9月～2011年3月从上海地区10个规模化养猪场分离的296株大肠埃希菌,采用微量肉汤稀释法进行药敏试验,采用PCR技术分别检测TEM、CTX- M、OXA和SHV基因。在296株大肠埃希菌中,有52株大肠埃希菌产ESBLs,产酶率为17.6%。在52株ESBLs菌株中,有46株为TEM型,占88.5%,有15株为CTX-M型,占28.8%,有11株为同时携带有TEM型和CTX-M型,占21.2%。大肠埃希菌产ESBLs株对10种常用抗菌药物的耐药性较为严重,而且存在多重耐药；上海地区猪源大肠埃希菌产ESBLs株流行的主要基因型为TEM型和CTX-M型。 |
| 关键词: 大肠埃希菌 超广谱β -内酰胺酶 耐药性 基因型 |
| DOI： |
| 投稿时间：2011-07-13修订日期：2011-07-29 |
| 基金项目: |
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| Analysis of ESBLs Genotype and Antimicrobial Resistance of ESBLs-Producing Escherichia Coli from Pigs |
| liuyani |
| (Shanghai Institute of Veterinary Drug and Feeds Control) |
| Abstract: |
| To investigate the isolation rate, antibiotic susceptibility and ESBLs′ genotypes of the Escherichia coli strains isolated from ten large-scale pig farms in Shanghai. 296 strains of Escherichia coli were collected between September 2010 to March 2011 and then and then susceptibility testing was performed by broth microdilution, and the genotypes of TEM, CTX-M, OXA and SHV genes were analyzed by PCR. There were 52 strains expressing ESBLs and the isolated rate was 17.6%. There were 46 strains expressing TEM gene (88.5%), 15 strains expressing CTX-M gene(28.8%) and 11 stains expressing TEM gene and CTX-M gene(21.2%) among 296 strains. The susceptibility testing against 10 antimicrobial agents of ESBLs producing Escherichia coli is serious and multidrug resistant. This study shows that TEM type and CTX-M type are predominant genotypes of ESBLs in Escherichia coli strains isolated from pigs. |
| Key words: Escherichia coli ESBLs resistance genotype |
