| 摘要: |
| 为鉴定维氏气单胞菌(Aeromonas veronii,AV)吉林分离株合适的诊断或免疫用抗原,本研究依据GenBank维氏气单胞菌外膜蛋白AⅠ基因(OMPAⅠ)设计引物,通过PCR成功扩增出长度为1026bp的基因片段,通过序列分析表明：该基因与维氏气单胞菌AB2902300 OMPAⅠ的同源性为93%,与其他气单胞菌同源性大于80%；同时通过生物信息学软件对其蛋白的分子特征进行了预测,为OMPAⅠ可能做为诊断或免疫用抗原提供了理论依据。 |
| 关键词: 维氏气单胞菌 外膜蛋白AⅠ 生物信息学 |
| DOI： |
| 投稿时间：2011-07-22修订日期：2011-07-22 |
| 基金项目:国家支撑计划项目（2010BAD04B01）；吉林省科技厅项目（20080218）；吉林农业大学青年启动基金 |
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| Cloning and bioinformatic analysis of outer membrane protein gene AⅠof Aeromonas veronii CY0806 from Cyprinus carpio |
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| Abstract: |
| To determine the proper diagnostic or immune antigen of Aeromonas veronii isolated from Jilin, the OMPA ? for 1026 bp was amplified by PCR using a couple of primers designed according to the gene sequence reported by GeneBank. The sequence analysis showed its homology with the OMPA ? of Aeromonas veronii AB2902300 reached 93% and others exceeded 80%. Meanwile, the molecular characteristics of protein was predicted by bioinformatics software and offered the theoretical basis for the OMPA ? as possible diagnostic or immune antigen. |
| Key words: Aeromonas veronii,OMPA ?,bioinformatics |
