| 摘要: |
| 摘要：建立HPLC法测定徐长卿中绿原酸的含量。采用索氏提取法以70%的甲醇水溶液为提取剂对徐长卿中的绿原酸进行提取。利用SHIMADZU C18色谱柱（250mm?4.6mm，5?m），流动相乙腈-0.5%磷酸水溶液（体积比1:9）；流速: 1.0mL?min-1；柱温:25℃；检测波长:327nm的条件进行检测。结果表明绿原酸在0.232μg～4.640μg范围内与峰面积呈良好线性关系, r=0.9997。平均回收率为99.32%，RSD为0.216%（n=5）。表明该方法简便、可靠、重现性好，可作为徐长卿中绿原酸含量测定的方法。 |
| 关键词: HPLC 徐长卿 绿原酸 |
| DOI： |
| 投稿时间：2011-07-24修订日期：2011-10-12 |
| 基金项目: |
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| Determination the Contents of Chlorogenic acid in Cynanchum paniculatum (Bge.) Kitag. with HPLC |
| YU Jia-ping |
| (College of Bio-engineering University of Agriculture S T of Jilin) |
| Abstract: |
| Abstract ： To establish for the determination the content of Chlomgenic acid in Cynanchum paniculatum (Bge.) Kitag..Using 70% methyl alcohol to extract Chlomgenic acid from Cynanchum paniculatum (Bge.) Kitag by Soxhlet extraction method. Methods with a HPLC method: The determination is measured by HPLC with SHIMADZU-C18(250mm ? 4.6mm ,5?m)column，Using acetonitrile - 0.5% H3 PO4(1:9) as mobile phase．The flow rate is 1.0mL?min-1，detection wave length is 327nm and Column temperature is 25 ℃. The results show that the linear range of chlomgenic acid is 0.232μg～4.640μg，r=0.9997．The average recovery is 99.32% with RSD 0.216%（n=5）. The method is simple and reliable with a good reproducibility which could be used to determine chlorogenic acid contents of Cynanchum paniculatum (Bge.) Kitag.. |
| Key words: HPLC Cynanchum paniculatum (Bge.) Kitag. Chlorogenic acid |
