| 摘要: |
| 建立了中药散剂中非法添加磺胺类药物检测的超高效液相色谱法。样品经甲醇提取、流动相稀释后进高效液相色谱仪进行检测分析。色谱条件：ACQUITY UPLC HSS T3(1.8μm,2.1?100mm)色谱柱,流动相为乙腈-0.017mol/L的磷酸溶液系统,检测波长范围200~340nm,柱温30℃,进样量为3μL,磺胺类药物在0.2~200μg/ml范围内呈良好的线性关系。该方法简便,准确,通过光谱比较,可以准确地判断中药中是否添加有磺胺类药物并进行准确地定量计算。 |
| 关键词: 中药散剂 磺胺类药物 超高效液相色谱 二极管阵列检测器 |
| DOI： |
| 投稿时间：2011-08-11修订日期：2011-08-31 |
| 基金项目: |
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| Determination of Sulfonamides in Traditional Chinese Medicine Powder by PLC-DAD |
| LIU Su-mei |
| (Henan Provincial Supervising Agency of Veterinary Drugs) |
| Abstract: |
| ABSTRACT: A method of UPLC-PDA for determination of Sulfonamides in traditional Chinese medicine was developed.The liquid chromatography separation was carried on ACQUITY UPLC HSS T3（1.8μm，2.1?100mm）with acetonitrile- 0.017mol/LH3PO4 system as mobile phase. The wavelength range was 190~400nm.The calibration curve was linear in the range of 0.2~200μg/mL. The method was simple,and would accurately detect Sulfonamides in traditional Chinese medicine powder. |
| Key words: Traditional Chinese Medicine Powder Sulfonamides HPLC DAD |
