| 摘要: |
| 建立了一种固相萃取-超高效液相色谱-串联质谱法快速测定猪尿中苯乙醇胺A的方法。猪尿样品以莱克多巴胺-D3为内标,尿液经酸化后离心,上清液经Oasis MCX固相萃取柱净化,超高效液相色谱-串联质谱法测定。苯乙醇胺A监测离子为m/z 345>150和m/z 345>327。结果表明在0.2~5 mg/L范围内苯乙醇胺A呈良好的线性关系,高中低浓度绝对回收率在80~110 %,精密度小于15 %,最低定量限0.2 mg/L。本方法快速、简便,适于猪尿中苯乙醇胺A的残留测定。 |
| 关键词: 猪尿 苯乙醇胺A 残留 固相萃取 UPLC-MS/MS |
| DOI： |
| 投稿时间：2011-09-29修订日期：2011-10-29 |
| 基金项目: |
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| Rapid determination of phenylethanolamine A in pig urine by SPE-UPLC-MS/MS |
| Bin Qu |
| (Jiangsu Quality Inspection and Testing Center for Animal Products) |
| Abstract: |
| A SPE-UPLC-MS/MS method was developed and established to rapidly determine phenylethanolamine A in pig urine. The urine sample was acidified and centrifuged, and the supernatant was cleaned up by Oasis MCX. UPLC-MS/MS was implemented to detect phenylethanolamine A by m/z 345>150 and m/z 345>327. The results illustrated that good linearity in the ranges of 0.2~5 mg/L for phenylethanolamine A. The absolute recoveries were between 80 % and 110 %, and precision was lower than 15 %. The limit of quantitative detection was 0.2 mg/L. The method was simple and rapid, and it was suitable to detect phenylethanolamine A residual in pig urine samples. |
| Key words: pig urine phenylethanolamine A residuals SPE UPLC-MS/MS |
