| 摘要: |
| 拟建立了癸氧喹酯原料药、固体分散体含量测定的高效液相色谱-紫外检测法。采用Diamonsil C18(2) 4.6?150 mm,5μm 色谱柱，以甲醇-水(95:5)溶液(用稀盐酸调pH值至3.5)为流动相，检测波长330 nm，流速为0.6 ml.min-1，外标法定量。结果显示该方法最低检测限为0.0025 μg.ml-1， 在0.5-10 μg.ml-1 浓度范围内，线性关系良好Y=11405X 15821，R2=0.9990，加样平均回收率99.61%，RSD为0.90%。方法可用于癸氧喹酯的含量测定，适用于该药品固体分散体生产的质量控制。 |
| 关键词: 高效液相色谱法 癸氧喹酯 含量测定 |
| DOI： |
| 投稿时间：2011-10-25修订日期：2011-11-28 |
| 基金项目: |
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| Study on determination of Decoquinate and its preparation by HPLC |
| jjiangfucheng |
| (Agriculture university of Hebei) |
| Abstract: |
| A detection method of HPLC-UV has been established for the determination of Decoquinate and its solid dispersion. A Diamonsil C18(2) column (4.6?150mm,5μm) was used with the mobile phase of methanol-water(95:5) solution(to adjust pH of 3.5 by dilute hydrochloric acid ),The UV detection wavelength was 330 nm, with a flow rate of 0.6 ml.min-1. The quantification was carried out by the external standard method. Results showed that the limit of detection of the method was 0.0025 μg.ml-1, The calibration curves have good linear over the range of 0.5~10 μg.ml-1 with the correlation coefficient of R2 =0.999 (n=6), The average recovery of Decoquinate was 99.61%, with RSD=0.90% (n=9). The all results were shown that the HPLC method may be used for the content determination of Decoquinate, applicable to the product quality control.. |
| Key words: HPLC decoquinate content determination. |
