| 摘要: |
| 芩花注射剂主要由黄芩、金银花、百部等组成。采取单因素试验筛选药物提取次数、提取时间/次、料液比，通过正交试验以绿原酸提取量为依据，最后确定提取次数为2次，每次提取1 h，料液比为1：10。采用HPLC法测定该注射液中黄芩苷和绿原酸含量，以峰面积(X)对进样浓度(Y)线性回归,黄芩苷回归方程：Y=2.22e 0.04X-7.67e 0.04 (r2 =0.999)。绿原酸回归方程：Y=2.57e 0.04X-6.53e 0.04 (r2 =0.998)，精密度、回收率、重复性、稳定性等指标均符合含量测定要求。本方法准确度好且操作简便。经测定，该注射液中黄芩苷和绿原酸的含量分别为3.292, 0.860 mg?mL－1。 |
| 关键词: 芩花注射剂 制备工艺 含量测定 |
| DOI： |
| 投稿时间：2011-11-28修订日期：2011-12-23 |
| 基金项目:黑龙江省博士后科研基金资助项目（LBH-Q05026） |
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| Preparation Technology and Content Determination of Qinhua Injection |
| LIU Ting |
| (College of Veterinary Medicine,Northeast Agricultural University) |
| Abstract: |
| Qinhua injection is mainly comprised of radix scutellariae, honeysuckle, radix stemonae. By single-factor experiment, the optimum extraction condition such as frequency, times, ratio of Chinese medicine to water were determined through orthogonal experiment based on the extracted chlorogenic acid, the result showed these Chinese medicines could be extracted twice by 10 times of water, 1 hour per time. HPLC method was performed to measure the content of baicalin and chlorogenic acid. The regress equations were Y=2.22e 004X-7.67e 004 (r2 = 0.999) and Y=2.57e 004X-6.53e 004 (r2=0.998), respectively. The indexes e.g. precision, recovery, repeatability and stability fitted in with the determination need, indicating that this HPLC method was sensiteve, time-saving and accurate. The contents of baicalin and chlorgenic acid in Qinhua injection were 3.292 and 0.860 mg?mL－1 , respectively. |
| Key words: Qinhua injection Preparation technology Content determination |
