| 摘要: |
| 建立了恩诺沙星、盐酸小檗碱粉中两种有效成分含量的高效液相色谱测定法。采用C18色谱柱（4.6 mm?250 mm，5 μm），检测波长265 nm，流动相为乙腈-0.005 moL/L庚烷磺酸钠溶液（用磷酸调节pH值至3.0）（40∶60），流速1.0 mL/min。结果显示，恩诺沙星在10.0～200.0 μg/mL范围内含量与峰面积呈良好的线性关系，相关系数为0.9999（n=6），平均回收率101.3%，RSD为2.0%；盐酸小檗碱在5.0～100.0 μg/mL范围内含量与峰面积呈良好的线性关系，相关系数为1.0000（n=6），平均回收率97.0%，RSD为2.6%。本方法简便、准确、快速、重现性好，可用于该制剂的含量测定。 |
| 关键词: 恩诺沙星 盐酸小檗碱 高效液相色谱法 |
| DOI： |
| 投稿时间：2011-12-26修订日期：2012-03-13 |
| 基金项目: |
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| Determination of Two Active Constituents in Enrofloxacin and Berberine Hydrochloride Powder by HPLC |
| CHEN Xiang-dan |
| (HuBei Veterinary Pharmaceutical Supervisory Centre,Hubei,Wuhan,430070) |
| Abstract: |
| An HPLC method was reported for the quantitative determination of two active constituents in enrofloxacin and berberine hydrochloride powder with C18 column（4.6 mm?250 mm，5 μm）. The detecting wavelength was 265nm; mobile phase was acetonitril-0.005 moL/L sodium heptanesulfonate (adjust to pH3.0 with phosphoric acid) (40∶60); flow rate was 1.0 mL/min. A good linear range of enrofloxacin and berberine hydrochloride was 10.0～200.0 μg/mL(r=0.9999,n=6) and 5.0~100.0 μg/mL (r=1.0000,n=6) respectively; the average recoveries was 101.3％(RSD=2.0％) and 97.0％(RSD=2.6％) respectively. This method was convenient, accurate, rapid, reproducible and suitable for the determination. |
| Key words: Enrofloxacin Berberine Hydrochloride HPLC |
