| 摘要: |
| 为了建立乳房炎涂膜剂中咖啡酸的反相高效液相色潽分析方法，试验采用Waters symmetry shieldTM C18 色谱柱（250 mm?4.6 mm，5 μm），流动相为甲醇 -1 %冰醋酸水溶液（20∶80）等度洗脱；流速：1.0 mL?min－1；检测波长323 nm；柱温为35 ℃。结果表明，咖啡酸在8 - 40 μg?mL－1 范围内线性关系良好（r2 =0.9989）。平均回收率为100.1 %，RSD为0.8 %。该方法简便，准确，可用于测定乳房炎涂膜剂中咖啡酸的含量。 |
| 关键词: 高效液相色谱 乳房炎涂膜剂 咖啡酸 |
| DOI： |
| 投稿时间：2012-04-11修订日期：2012-05-06 |
| 基金项目:黑龙江省博士后科研基金资助项目（LBH-Q05026） |
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| Determination of Caffeic Acid in Mastitis Plastic by HPLC |
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| (College of Veterinary Medicine,Northeast Agricultural University) |
| Abstract: |
| A method was established for the determination of caffeic acid in mastitis plastic by Reversed Phase High Performance Liquid Chromatography (RP-HPLC) . Chromatography was performed on a Waters symmetry shieldTM C18 column(250 mm?4.6 mm，5 μm) at 323 nm and 35℃. The mobile phase consisted of a mixture of methanol-acetic acid (20∶80) at a flow rate of 1.0 mL?min－1. The results showed that there were good linear relations between the concentrations and the peak-areas of caffeic acid with the range of 8 - 40μg?mL－1. The standard solution was stable in 16 h（RSD=0.6 %）. The average recovery was 100.1 % (RSD=0.8 %). This method is simple, accurate, and can be used for the determination of caffeic acid in mastitis plastic. |
| Key words: HPLC mastitis plastic caffeic acid |
