| 摘要: |
| 肺炎支原体的培养和保存条件非常苛刻，是疫苗规模化生产的工艺难题。本文针对猪支原体肺炎活疫苗（168株）生产工艺关键技术进行研究。通过培养试验筛选四种培养基配方，CCU测定证明该疫苗株在低血清改良培养基中生长良好；优化发酵培养工艺，使其在发酵罐培养60~70 h的峰值可达到1010 CCU/mL；设计筛选168株耐热保护剂和冻干工艺，37℃下保存10d的耐老化试验结果显示，下降滴度小于10 0.5 CCU/mL。本研究为提供高效、安全和稳定的猪肺炎支原体疫苗产品奠定基础。 |
| 关键词: 猪肺炎支原体 改良培养基 发酵工艺 耐热保护剂 |
| DOI： |
| 投稿时间：2012-05-25修订日期：2012-06-25 |
| 基金项目:江苏省科技成果转化专项资金BA2010021，江苏省农业科技自主创新资金CX（11）2059 |
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| Study on the Key Production Process of Mycoplasma hyopneumoniae, Live Vaccine (168 strain) |
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| (Nanjing Tianbang Bio-Industry Co Ltd) |
| Abstract: |
| It is strictly hard for culturing and storage of Mycoplasma hyopneumoniae (Mhp),which is the technical problem of vaccine integrated production. We screened four kind of culture medias of Mhp, and the color-changing units (CCU) detection results showed that Mhp strain 168 grew well in low-serum modified medium. For the optimization of the fermentation technology, the peak titers of highest growth, of strain 168, were 1010CCU /mL after cultured 60~70h in fermentor. With optimization of heat resisting protective and lyophilization technology, the titers of strain 168 were only reduced 100.5CCU /mL during the aging tolerance test which lasted for 10 days at condition of 37℃. This study built the foundation for the efficient, safety and stabile producing the Mhp live vaccines. |
| Key words: Mycoplasma hyopneumoniae live vaccine modified medium fermentation technology heat resisting protective |
