| 摘要: |
| 依据GenBank中登录的常见细胞污染支原体16S rRNA基因序列，选择高度保守的区域设计引物，建立了一种快速灵敏的支原体PCR检测方法。该方法可以特异性检测出6种支原体，并能敏感的检测到10个拷贝数的目的基因。采用建立的PCR方法和传统的培养法对23个不同样品（细胞、血清、疫苗成品、半成品）进行检测，符合度100％。该方法的建立可大大缩短疫苗生产过程中支原体检验所需时间。 |
| 关键词: 支原体 PCR方法 培养法 |
| DOI： |
| 投稿时间：2012-09-13修订日期：2013-01-07 |
| 基金项目: |
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| Establishment and Application of a PCR Method for the Detection of Mycoplasma |
| 巢伟,徐 雷 |
| (Hunan Sinoland Bio-Pharmacy Co., Ltd) |
| Abstract: |
| According to the 16S rRNA gene sequence published in GenBank coming from common cell culture contaminant Mycoplasma, a pair of primers from highly conserved nucleotide sequences was designed, and a rapid and sensitive PCR method for detection of Mycoplasma was established. Six reference Mycoplasma strains were correctly detected by PCR method. It can sensitive detect 10 copies templates. The result-agreement rate of culture method and PCR method for 23 different samples (including serum, cell, vaccine products and semi-finished products) were 100%. This method can greatly reduce the detection time of Mycoplasma in vaccine production process. |
| Key words: Mycoplasma PCR method culture method |
