| 摘要: |
| 建立了吡喹酮-聚乳酸-羟基乙酸（吡喹酮-PLGA）缓释植入棒含量测定的HPLC法，并对其释放度进行了考察。以甲醇-水（100:40，V/V）为流动相，采用Grace C18反相色谱柱（ 4.6mm?250mm，5μm），流速1.0mL/min，紫外检测波长263nm，在此试验条件下，吡喹酮在10～200μg/mL范围内与峰面积线性关系良好，r=0.9999；平均回收率为99.48%，RSD 为0.53%（n=9）。三批样品的含量分别为98.96%、98.67%和98.81%，该方法简单、快捷、辅料无干扰、准确度高，适于吡喹酮-PLGA植入棒的含量测定。吡喹酮-PLGA植入棒以骨架溶蚀释放机制缓慢释放，释药期可达三周，释放度高于300μg/d。 |
| 关键词: 吡喹酮 PLGA 缓释植入棒 含量测定 HPLC 释放度 |
| DOI： |
| 投稿时间：2012-11-20修订日期：2013-03-19 |
| 基金项目: |
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| Determination and Release rate of Veterinary Sustained-release Praziquantel-PLGA Implants |
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| (Shandong Provincial Key Laboratory of Biomedical Polymers,Shandong Institute of Medical Instrumens) |
| Abstract: |
| The paper estabalisted an HPLC method for determination of the praziquantel-PLGA implants, and investigate release rate. The diamonsil C18 column was used, the mobile phase consisted of methanol and ultrafiltrated water (100:40, v/v), the flow rate was 1.0mL/min, the detection wavelength was at 263nm. Praziquanel has a good linearity at the range of 10μg/mL～200μg/mL(r=0.9999), and the average recovery was 99.48% with RSD 0.53%(n=9). The determinations of three lots samples are 98.96%, 98.67% and 98.81%, respectively. Praziquantel can be slowly released from the implant to three weeks and release rate is more than 300μg/d. The method is simple, rapid and accurate without excipients interference, and suitable for the determination of the praziquantel-PLGA implants. Praziquantel can be slowly released with the frame-erosion mechanism. |
| Key words: praziquantel PLGA sustained-release implant determination HPLC release rate |
