| 摘要: |
| 为了大规模生产猪传染性胃肠炎活疫苗抗原，试验采用生物反应器及微载体进行ST细胞的培养，待微载体上的ST细胞长满至单层后接种猪传染性胃肠炎病毒（TGEV）。共使用生物反应器培养3批TGEV抗原，每批培养过程中分别调节初始细胞密度至2.14?106 个/mL、1.83?106 个/mL和2.02?106 个/mL，微载体浓度为3 g/L/、6 g/L和9 g/L。结果表明应用生物反应器及微载体培养得到抗原的病毒含量均达到108.0 TCID50/mL，明显高于转瓶培养的病毒含量。 |
| 关键词: 传染性胃肠炎病毒 生物反应器 微载体 ST细胞 |
| DOI： |
| 投稿时间：2013-03-13修订日期：2013-05-08 |
| 基金项目: |
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| Cultivation of TGEV in Bioreactor |
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| (Sichuan Huapai Biopharmceutical Co.,Ltd.) |
| Abstract: |
| To get the Transmissible gastroenteritis virus(TGEV)in large scale, ST cells were cultured in bioreactor with microcarrier, when the cells on microcarrier grew to monolayer, TGEV were inoculated into cell culture. The bioreactor was used for three times, the cell density on microcarriers were 2.14×106 /mL、1.83×106 /mL and 2.02×106 /mL respectively, concentration of microcarriers were 3 g/L/、6 g/L and 9 g/L respectively. The results show that the titers of TGEV cultured in bioreactor can reach to 108.0 TCID50/mL, which is higher than that in roller bottles. |
| Key words: TGEV bioreactor microcarrier ST cells |
