| 摘要: |
| 原位杂交技术自上世纪80年代发展以来，由于可在保存组织细胞完整性的同时于原位定位基因表达的产物，在科学研究中已有广泛应用。根据探针标记方法的差异，对原位杂交技术在兽医微生物研究中各个阶段的技术原理、应用现状、发展前景以及存在的不足进行了综述。 |
| 关键词: 原位杂交技术 QuantiGene ViewRNA 原位杂交技术 |
| DOI： |
| 投稿时间：2013-04-17修订日期：2013-06-27 |
| 基金项目:新疆维吾尔自治区科技支疆项目计划《新疆部分种猪场猪瘟净化技术的集成研究与示范》（201291150） |
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| Application progress of In Situ Hybridization Technology in veterinary microbiology |
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| (China Institute of Veterinary Drug Control) |
| Abstract: |
| In Situ Hybridization (ISH) technique was invented and developed in 80s which can position gene expression products in situ without destroying tissue integrity, thus have been widespread used in scientific study. Based on different probe marking methods, different ISH technologies’ technical principles, current applications, prospects and disadvantages are elaborated in the veterinary microbiology . |
| Key words: In Situ Hybridization QuantiGene○R View RNA ISH. |
