| 摘要: |
| 为了研究能有效预防锦鲤疱疹病毒的疫苗，本文研究克隆了锦鲤疱疹病毒ORF136基因，并构建重组质粒PET32a-ORF136，用IPTG诱导表达，SDS-PAGE检测获得含his标签大小为36.4kDa的蛋白条带。蛋白纯化后免疫鲤鱼，不同天数采血收集血清用间接ELASA检测抗体水平。结果显示：用10ug/尾、20ug/尾和40ug/尾的蛋白免疫后均可检测到KHV的抗体，用PET32a-ORF136表达的蛋白20ug/尾和40ug/尾的蛋白免疫后的抗体水平比10ug/尾剂量蛋白免疫后的抗体水平高但差异不显著（p＞0.05）。 |
| 关键词: 锦鲤疱疹病毒 ORF136基因 原核表达 免疫原性 |
| DOI： |
| 投稿时间：2013-06-21修订日期：2013-08-21 |
| 基金项目:基金项目：长春市科学技术局先进实用技术的示范推广项目（12XN35） |
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| Immunity Test Research and Prokaryotic Expression of ORF136 Gene of Koi Herpesvirus -CJ Strain |
|
| (Jilin Agricultural University) |
| Abstract: |
| To study a vaccine which can be used to effectively prevent Koi herpes virus , Koi herpes virus ORF136 gene was selected, the recombinant plasmid PET32a-ORF136 was induced by IPTG, SDS -PAGE detection of the protein indicate the size of 36.4kDa bands（Contains his Tags）.Purified protein immuned carps, and serum was collected in different days, antibodies level of serum were determined by indirect ELISA.T The results show that health koi can induce the production of KHV antibodies after immune proteins at 10μg/fish, 20μg/fish and 40μg/fish were intramuscular injected into health kois, respectively .The antibody levels of the group of 20μg/fish and the group of 40μg/fish were higher than the group of 10μg/fish, and the differences were not significant (p>0.05). |
| Key words: Koi herpes virus, ORF136gene, Prokaryotic Expression, Immunogenicity |
