| 摘要: |
| 以传染性法氏囊病病毒(IBDV)BC6/85株基因组RNA为模板,采用RT-PCR方法扩增并克隆了IBDV BC6/85株基因组全长cDNA。序列测定结果表明：A节段全长共3260个核苷酸,与IM株的同源性最高为97.4%,与其他血清I型毒株的核苷酸同源性介于91.2% ~97.1%；B节段有2827个核苷酸,与IM株同源性最高为98.6%,与其它毒株的核苷酸同源性为88.7%~98.2%。通过对编码的氨基酸序列进行分析,发现BC6/85株有21个特有氨基酸,其中15个位于多聚蛋白VP2-4-3。系统进化树分析表明,BC6/85株与经典毒株、弱毒株和变异株的关系较近,而与超强毒株相对较远。 |
| 关键词: 传染性法氏囊病病毒 全基因组 序列分析 |
| DOI： |
| 投稿时间：2013-06-28修订日期：2013-07-23 |
| 基金项目: |
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| Sequence Analysis of the Complete Genome of IBDV BC6/85 |
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| (China Institute of Veterinary Drug Control) |
| Abstract: |
| The full-length of double segments of the infectious bursal disease virus (strain BC6/85) was amplified by RT-PCR and sequenced. The result showed that segment A contains 3260bp,compared with other IBDV strains,BC6/85 segment A shared 91.2%~97.4% homology at nucleotide level,segment B contains 2827bp,which shared 88.7%~98.6% homology. The analysis of deduced amino acid sequences indicated that IBDV BC6/85 strain has 21 specific residues. Phylogenetic tree analysis indicated BC6/85 is more closely related to the attenuated, classical and variant IBDV than those of very virulent IBDV strains. |
| Key words: infectious bursal disease virus complete genome sequence analysis |
