| 摘要: |
| 以大肠杆菌表达、纯化的禽白血病病毒(ALV)重组P27蛋白为包被抗原，建立了检测ALV-P27 抗体的间接ELISA方法，为禽白血病流行病学调查提供了一种简便的血清学检测方法。该方法与9种禽常见传染病病毒阳性血清及大肠杆菌阳性血清无交叉反应，具有较好的特异性；批内、批间重复试验变异系数均小于10％，具有良好的重复性；新建立的ELISA方法比IDEXX公司生产的ALV A、B亚群抗体检测试剂盒和J亚群抗体检测试剂盒相对于免疫荧光试验（IFA）有更高的符合率。 |
| 关键词: 禽白血病病毒 重组P27蛋白 间接ELISA |
| DOI： |
| 投稿时间：2013-07-03修订日期：2013-09-25 |
| 基金项目: |
|
| Development and Preliminary Application of an Indirect-ELISA Diagnostic Method for Detection of Avian Leukosis Virus Antibodies |
|
| (China Institute of Veterinary Drug Control,Beijing,100081) |
| Abstract: |
| An indirect-enzyme-linked immunosorbent assay(iELISA) method was developed for detecting antibodies against Avian leukosis virus (ALV) using recombinant P27 protein. There were no cross reaction with antiserum against 9 kinds of avian infectious disease agent and antiserum against E.coli.Variation coefficients were less than 10% in the intro-batch and in inter-batch tests.The protocol of iELISA was optimized and the method showed more sensitive and specificity than the commercial IDEXX ALV antibody test kit when immunoflourescentassay(IFA)was used as a reference．The results showed that the developed iELISA was specific and can potentially be used for detection of anti-ALV antibodies on a large scale. The indirect ELISA assay would provide a simple and rapid means of monitoring ALV infection． |
| Key words: avian leukosis virus recombinant P27 protein indirect-ELISA |
