| 摘要: |
| 鸡黄病毒FQ-C1株经SPF鸡胚连续传40代后，其尿囊液毒的ELD50达到1?10－4.33/0.1 mL。将该病毒第40代SPF鸡胚液毒灭活后制备成油乳剂灭活疫苗，SPF雏鸡安全性试验和蛋鸡安全性试验表明该疫苗的安全性好，无不良影响。以该疫苗一次单剂量（1 mL/只）免疫开产蛋鸡28 d后攻以鸡黄病毒强毒测定疫苗的保护效果，结果显示疫苗免疫组较未免疫组蛋鸡的产蛋率高出75.8%，产蛋保护率达93.2%以上，表明本研究制备的鸡黄病毒灭活疫苗安全性好，且具有良好的免疫原性，免疫后可有效抵抗鸡黄病毒所致的产蛋骤降。 |
| 关键词: 鸡黄病毒 灭活疫苗 免疫效果测定 |
| DOI： |
| 投稿时间：2013-08-26修订日期：2013-11-01 |
| 基金项目:新世纪“百千万人才工程”国家级人选科研补助资金（NCNCMTPC-2009）、福建省省属公益类项目(2011R1025-2)。 |
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| Preparation and Protective Efficacy of Inactivated Vaccine of Chicken Flavivirus |
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| (Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences) |
| Abstract: |
| After serially passaged in specific pathogen free embryonated chicken eggs, the chicken flavivirus strain FQ-C1 was stable at a titer of 1×104.33 ELD50 per 0.1 mL after 40 passages. Inactivated with formaldehyde, the passaged virus of strain FQ-C1 emulsified with mineral oil adjuvant in an appropriate ratio, and then inactivated vaccine is made. We test the biosecurity of the inactivated oil-emulsion vaccines through immunization in 1-day-old chicken and laying hens. The results showed that animals immunized with the inactivated vaccines appear no adverse effects. Twenty-eight days after administration of inactivated vaccine with a dose of 1 mL per chicken, the laying hens attacked by a virulent chicken flavivirus. The results showed that the laying rate of hens immunized with vaccine was 75.8% higher than that of control group, and the protective efficacy of the inactivated vaccine on egg production was no less than 93.2%. All of the results indicated that the inactivated oil-emulsion vaccine had good immunogenicity, which could effectively resist egg-dropping caused by chicken flavivirus. |
| Key words: chicken flavivirus inactivated vaccine protective efficacy |
