| 摘要: |
| 为评价布鲁氏菌病补体结合酶联免疫吸附试验抗体检测试剂盒（CF-ELISA试剂盒）的敏感性、特异性及与其它方法的符合率。用CF-ELISA试剂盒对布病感染地区牛、羊血清各200份，布病净化地区牛、羊群血清各100份进行抗体检测，同时与其他商品化检测试剂进行了比较。结果表明，感染地区牛、羊血清抗体的McNemar检验结果表明CF-ELISA试剂盒与iELISA 试剂盒和CGS的敏感性和特异性差异均不显著(P＞0.05)，Kappa一致性检验分析结果表明，CF-ELISA试剂盒与iELISA试剂盒检测结果有高度的符合性。检测净化地区牛、羊群血清抗体产品间的特异性和符合率均为100%。 |
| 关键词: 布鲁氏菌病 CF-ELISA iELISA RBT CGS |
| DOI： |
| 投稿时间：2013-10-24修订日期：2013-12-26 |
| 基金项目:公益性行业(农业)科研专项：布鲁氏菌病、牛结核病防控技术研究(NO. 200903027) |
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| Evaluation of the sensibility and specificity of the complement fixation enzyme linked immunosorbent assay kit for detecting brucellosis antibodies |
| 王楠,毛开荣 |
| (China Institute of Veterinary Drug Control) |
| Abstract: |
| To analysis the sensibility, specificity and coincidence rate of the complement fixation enzyme linked immunosorbent assay kit（CF-ELISA kit）for detecting brucellosis antibodies. Select CF-ELISA kit to detect brucellosis antibodies of 400 bovine and sheep serum specimens were collected from brucellosis-affected areas, where cases have already been identified. Another total of 200 bovine and sheep serum specimens for detecting antibodies were collected from no brucellosis-affected areas, where no cases have been identified. Results show that there was no significant difference (P>0.05) between the CF-ELISA kit to iELISAkit and CGS for detecting antibodies of brucellosis-affected areas serum. Kappa statistics result shows that the method of CF-ELISA and iELISA have highly coincidence rate. The specificity and coincidence rate were 100% between the CF-ELISA kit and iELISA kit for detecting antibodies of no brucellosis-affected areas serum. |
| Key words: Brucellosis CF-ELISA iELISA RBT CGS |
