| 摘要: |
| 为准确掌握猪瘟疫苗免疫效果，2010年下半年至2012年上半年对投放安徽省的猪瘟疫苗免疫效果进行了监测。采用抗体阻断ELISA方法，检测了454个猪场7439份免疫猪瘟弱毒活疫苗1个月左右的血清中猪瘟免疫抗体水平。结果显示，我省猪瘟疫苗免疫效果总体良好，免疫抗体合格率超过了农业部要求的70%以上的标准；脾淋组织苗和细胞苗免疫效果相当，但脾淋组织苗免疫效果稳定，细胞苗免疫效果稳定性较差；不同厂家疫苗免疫效果参差不齐。 |
| 关键词: 猪瘟疫苗 免疫效果 监测 抗体阻断ELISA |
| DOI： |
| 投稿时间：2013-12-03修订日期：2014-01-20 |
| 基金项目: |
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| A Monitoring of the Field Immunity Efficacy of Classical Swine Fever |
|
| (Anhui Animal Disease Control Center) |
| Abstract: |
| In order to know well the immunity efficacy of classical swine fever(CSF) vaccine, from the later half of 2010 to the first half of 2012, we had monitored the immunity efficacy of classical swine fever vaccine which was used in Anhui province. 7439 swine serums collected from 454 swine farms that had been immunized with CSF vaccine for one month or so had been detected for the antibody titer of CSFV by antibody blocking ELISA. The results indicated that the immunity efficacy of CSF vaccine was good, and the positive rate of immune antibody exceeded 70% which was demanded by MOA of China. The immunity efficacy of CSF vaccine of both spleen and lymph tissue origin and tissue culture origin was not much different, but the immunity efficacy of spleen and lymph tissue origin was steady, and the tissue culture origin was unsteady. Furthermore, the immunity efficacy of CSF vaccine from different manufactures was different. |
| Key words: classical swine fever vaccine, immunity efficacy, monitoring,antibody blocking ELISA |
