| 摘要: |
| 本文建立了鸡肉中尼卡巴嗪残留标志物4,4’-二硝基均二苯脲(DNC)残留酶联免疫(ELISA)检测方法。将N-琥珀酰-L-丙氨酰-L-丙氨酰-L-丙氨酸4-硝基苯胺(SAN)与载体蛋白偶联作为抗原免疫动物,获得抗DNC抗体,在此基础上建立了鸡肉中尼卡巴嗪残留的检测方法。人工抗原中SAN与载体蛋白的结合比约为12:1,血清效价1:2000倍,鸡肉中检测限为9.2 μg/kg,添加回收率在49.4%~118%之间,批内、批间变异系数均小于20%。该方法在灵敏度、精密度和准确度方面均能满足兽药残留筛选检测要求。 |
| 关键词: 尼卡巴嗪 4,4’-二硝基均二苯脲 残留 鸡肉 酶联免疫法 |
| DOI： |
| 投稿时间：2013-12-05修订日期：2014-02-27 |
| 基金项目:国家科技支撑计划（2011BAK10B01-11） |
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| Enzyme-linked immunosorbent assay for detection of nicarbazin residue in chicken muscle |
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| (China Institute of Veterinary Drug Control) |
| Abstract: |
| ELISA for detection of 4,4’-dinitrocarbanilide（DNC），marker residue of nicarbazin，in chicken muscle was developed. Anti-DNC antibody was gotten by immunizing animals with SAN-protein combination. The conjugate rate of SAN and protein was 12:1 in artificial antigen. Antiserum valence was more than 1:2000. The limit of detection was 9.2 μg/kg in chicken muscle. The recovery rate range was from 49.4% to 118%. The sensibility，precision and accuracy of the method could satisfy the request of screening veterinary drug residue. |
| Key words: nicarbazin DNC residue chicken muscle ELISA |
